{"Id":"0df273ff-4091-4853-8b60-1eef8bee714b","CanonicalId":"","Url":"https://pubmed.ncbi.nlm.nih.gov/30271983/","Name":"cPRISM-SRM (carrier-assisted high-pressure, high-resolution separations with intelligent selection and multiplexing coupled to selected reaction monitoring)","Description":"cPRISM-SRM is an extension of MS-based proteomic analysis with the key feature of being able to characterize low numbers of mammalian cells. Compared to current targeted MS methods,  CPRISM-SRM requires 3–4 orders of magnitude lower cell numbers. The method capitalizes on using \"\"carrier protein\"\" to assist processing of low numbers of cells with minimal loss, high-resolution PRISM separation for target peptide enrichment, and sensitive-targeted MS (SRM) for protein quantification. cPRISM-SRM has sufficient sensitivity to quantify proteins expressed at ≥200,000 copies per cell at the single-cell level and\u2009≥3000 copies per cell in 100 mammalian cells. With further improvement cPRISM-SRM has the potential to move toward targeted MS-based single-cell proteomics.","ResourceType":"AssayTechnology","ParentResourceType":"Technology","DefiningManuscriptId":"PMID30271983","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"tujin_shi@hirnetwork.org","FirstName":"Tujin","LastName":"Shi","EmailAddress":"tujin.shi@pnnl.gov","Organization":"Pacific Northwest National Laboratory","Consortia":["CBDS"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"wei-jun_qian@hirnetwork.org","FirstName":"Wei-Jun","LastName":"Qian","EmailAddress":"weijun.qian@pnnl.gov","Organization":"Pacific Northwest National Laboratory","Consortia":["CBDS","OPP"]}],"Vendor":{"id":null,"Name":"","Url":""},"VendorResourceName":"","VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2019-04-26T01:22:19.1197314+00:00","DateModified":"2022-07-01T03:35:02.3649529+00:00","VersionId":"1","ModelVersion":"4.0","TechnologyData":{"Dependencies":[],"AssayTechnologyData":{"BioSampleType":[],"CellsTestedWith":[{"CanonicalId":"http://purl.obolibrary.org/obo/CL_0002327","Url":"http://purl.obolibrary.org/obo/CL_0002327","Name":"mammary gland epithelial cell","OntologyId":"CL"}],"AssayTarget":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"AssayCategory":{"CanonicalId":"http://purl.obolibrary.org/obo/OBI_0000470","Url":"http://purl.obolibrary.org/obo/OBI_0000470","Name":"mass spectrometry assay","OntologyId":"OBI"},"TechnologyType":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"TechnologyPlatform":"","CompatibleTools":[]},"DeviceEquipmentTechnologyData":{"CompatibleTools":[],"CellsTestedWith":[],"Placeholder":"Test"},"ServiceTechnologyData":{"CellsTestedWith":[],"Placeholder":"test"},"CodePipelineTechnologyData":{"Placeholder":"Test"}}}