{"Id":"2d86643e-daae-11e7-9bda-db9b5c1a3f04","CanonicalId":"RRID:SAMN47586611","Url":"","Name":"human antigen-specific CD8+ T-cells","Description":"These engineered T cells are autoreactive and human beta cell-specific, and have been used in chromium release assays with human islets as targets. The CD8+ T cells were isolated from healthy donor PBMCs and transduced with lentiviral vectors containing TCR expression constructs pCCL.IGRPopt.eGFP recognizing the autoantigen IGRP or LV.Mart1.TCR.RK recognizing the non-beta-cell antigen MART1, then expanded.","ResourceType":"PrimaryCell","ParentResourceType":"Cell","DefiningManuscriptId":"PMID28877912","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"todd_brusko@hirnetwork.org","FirstName":"Todd","LastName":"Brusko","EmailAddress":"tbrusko@ufl.edu","Organization":"University of Florida","Consortia":["CMAI","OPP"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"mark_atkinson@hirnetwork.org","FirstName":"Mark","LastName":"Atkinson","EmailAddress":"atkinson@pathology.ufl.edu","Organization":"University of Florida","Consortia":["CBDS","HPAP","OPP"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"clayton_mathews@hirnetwork.org","FirstName":"Clayton","LastName":"Mathews","EmailAddress":"cxm@ufl.edu","Organization":"University of Florida","Consortia":["CMAI","CBDS","OPP"]}],"Vendor":{"id":null,"Name":"","Url":""},"VendorResourceName":"","VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2017-12-06T17:52:08+00:00","DateModified":"2022-07-01T03:36:32.7514303+00:00","VersionId":"4","ModelVersion":"4.0","CellData":{"CellType":[{"CanonicalId":"http://purl.obolibrary.org/obo/CL_0000625","Url":"http://purl.obolibrary.org/obo/CL_0000625","Name":"CD8-positive, alpha-beta T cell","OntologyId":"CL"}],"CellMarkers":[],"CultureConditions":"","Species":[{"CanonicalId":"http://purl.bioontology.org/ontology/STY/T016","Url":"http://purl.bioontology.org/ontology/STY/T016","Name":"Human","OntologyId":"NCBITAXON"}],"Sex":null,"Ethnicity":[],"PrimaryDisease":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"SecondaryDisease":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"SpecimenState":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"AgeAtSampling":"","AgeAtDiseaseOnset":"","AgeAtDeath":"","TransientModifications":"","DifferentiatedCellData":{"PrecursorCell":"","GeneticModifications":"","PassageNumber":""},"OtherCellLineData":{"GrowthProperties":"","PrecursorLineName":"","InductionMethod":"","PassageNumber":"","AuthenticationMethod":"","AuthenticationFrequency":"","AuthenticationResult":""},"PrimaryCellData":{"SourceTissue":[],"InductionMethod":""},"StemCellData":{"PassageNumber":"","PassagingMethod":"","InductionMethod":"","KaryotypingResult":"","PassageLastKaryotyping":"","ReprogrammingVectorType":"","ReprogrammingGeneCombo":"","OtherReprogrammingConditions":""}}}