{"Id":"2f12e8ad-255c-4691-b4c9-2938cbfe94a1","CanonicalId":"GSE151878","Url":"https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151878","Name":"Cardiomyocytes Infected by SARS-CoV-2 Recruit Cardiotoxic Macrophages [scRNA-seq dataset]","Description":"Heart injury has been reported in up to 20% of COVID-19 patients, yet the cause of myocardial histopathology is unknown. We examined heart tissue from autopsies of healthy and COVID-19 patients using RNA-seq and identified a strikingly increased CCL2 expression and macrophage population in COVID-19 heart sample. Transwell assays using hESC-derived cardiomyocytes (CMs) and macrophages revealed that SARS-CoV-2 infected CMs secrete CCL2, which recruits macrophages, and this was validated using adult human CMs. Macrophages recruited by CCL2 from infected CMs secrete IL-6 and TNF-α, which causes increased ROS and cell apoptosis of CMs. Finally, a high content chemical screen using FDA-approved drugs identified ranolozine and tofacitinib, which rescue SARS-CoV-2 infected CMs from macrophages-induced cardiotoxicity.","ResourceType":"Dataset","ParentResourceType":null,"DefiningManuscriptId":"PMID34403650","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":null,"Name":"Producer","OntologyId":null},"HirnUserId":"peng_wang@hirnetwork.org","FirstName":"Peng","LastName":"Wang","EmailAddress":"peng.wang@mssm.edu","Organization":"Mount Sinai Hospital","Consortia":["CTAR"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":null,"Name":"Producer","OntologyId":null},"HirnUserId":"shuibing_chen@hirnetwork.org","FirstName":"Shuibing","LastName":"Chen","EmailAddress":"shc2034@med.cornell.edu","Organization":"Weill Cornell Medical College","Consortia":[]}],"Vendor":{"id":null,"Name":"","Url":null},"VendorResourceName":null,"VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2022-01-13T21:50:22.9211747+00:00","DateModified":"2022-07-01T03:37:21.3731103+00:00","VersionId":"1","ModelVersion":"4.0","DatasetData":{"DatasetType":{"CanonicalId":"http://purl.bioontology.org/ontology/NCIT?conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C153189","Url":"http://purl.bioontology.org/ontology/NCIT?conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C153189","Name":"Transcriptomics Dataset","OntologyId":"NCIT"},"ExperimentDesign":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"BiosampleCharacteristics":[],"BiosampleType":[],"TechnologyType":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null}}}