{"Id":"3cd0c925-0235-4920-bf0c-ba63a41d2312","CanonicalId":"dx.doi.org/10.17504/protocols.io.bvyyn7xw","Url":"https://www.protocols.io/view/electrophysiological-characterizations-of-pancreat-bvyyn7xw","Name":"Electrophysiological Characterizations of Pancreatic Islet Cells","Description":"Hormone release from pancreatic cells, for example glucagon from α-cells and insulin from β-cells, is controlled by the cells’ membrane potentials (Vm), which are in turn finely controlled by multiple classes of ion channels, transporters, and pumps. The perforated whole-cell patch-clamp method represents a direct and well-established way to monitor Vm in individual cells and also to investigate ionic currents (Im) that contribute to determination of Vm. Here a step-by-step guide to obtain various patch-clamp measurements from pancreatic cells in isolated intact human islets is provided. The method requires a standard electrophysiological station and β escin is used as the perforating agent. Select exemplar current-clamp Vm results and voltage-clamp Im results are provide. The electrophysiological characteristics may be used to distinguish pancreatic cell types, for example α-cells vs. β-cells in real time.","ResourceType":"Protocol","ParentResourceType":null,"DefiningManuscriptId":"","Publications":[],"Contributors":[],"Vendor":{"id":null,"Name":"","Url":null},"VendorResourceName":null,"VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2021-12-06T19:00:22.1700158+00:00","DateModified":"2022-07-01T03:36:55.5970976+00:00","VersionId":"1","ModelVersion":"4.0","ProtocolData":{"ProtocolType":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"DocumentId":null,"ProtocolSpecies":[],"DocumentTopic":[]}}