{"Id":"5f5707ce-d488-4d9b-b075-fc1a0b0adaaf","CanonicalId":"","Url":"https://pubmed.ncbi.nlm.nih.gov/29434325/","Name":"CellCycleTRACER (mass cytometry assay method for deconfounding cell-cycle and cell-volume effects)","Description":"CellCycleTRACER is a combined experimental and computational method to account for the often confounded factors cell cycle and cell volume in mass cytometry data. Resolving cell heterogeneity caused by either cell-cycle state or cell volume in single-cell transcriptomic data has relied on statistical analysis using known cell-cycle genes. For mass cytometry, there is so far a more limited selection of available informative markers related to cell cycle and volume. As an assay method, CellCycleTRACER exploits four cell-cycle markers recently identified by Behbehani et al. 2012 (phosphorylated histone H3, phosphorylated retinoblastoma,  cyclin B1, and 5-Iodo-2′-deoxyuridine) to distinguish cell populations in different cell-cycle states. For measuring cell volume, CellCycleTRACER uses the ruthenium complex ASCQ_Ru. CellCycleTRACER may be particularly important when heterogeneous cell populations with deregulated cellular processes, as typically found in tumors, are analyzed.","ResourceType":"AssayTechnology","ParentResourceType":"Technology","DefiningManuscriptId":"PMID29434325","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"bernd_bodenmiller@hirnetwork.org","FirstName":"Bernd","LastName":"Bodenmiller","EmailAddress":"bernd.bodenmiller@imls.uzh.ch","Organization":"University of Zurich","Consortia":["CBDS"]}],"Vendor":{"id":null,"Name":"","Url":""},"VendorResourceName":"","VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2019-04-26T01:22:19.1197314+00:00","DateModified":"2022-07-01T03:35:02.3642503+00:00","VersionId":"1","ModelVersion":"4.0","TechnologyData":{"Dependencies":[],"AssayTechnologyData":{"BioSampleType":[],"CellsTestedWith":[],"AssayTarget":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"AssayCategory":{"CanonicalId":"http://purl.obolibrary.org/obo/OBI_0001977","Url":"http://purl.obolibrary.org/obo/OBI_0001977","Name":"cytometry assay","OntologyId":"OBI"},"TechnologyType":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"TechnologyPlatform":"","CompatibleTools":[]},"DeviceEquipmentTechnologyData":{"CompatibleTools":[],"CellsTestedWith":[],"Placeholder":"Test"},"ServiceTechnologyData":{"CellsTestedWith":[],"Placeholder":"test"},"CodePipelineTechnologyData":{"Placeholder":"Test"}}}