{"Id":"729e50e6-46e2-4638-8ba6-c60a3221c033","CanonicalId":"","Url":"","Name":"A versatile, portable intravital microscopy platform for studying beta-cell biology in vivo ","Description":"The platform includes engineered beta-cell-selective virally-encoded fluorescent protein biosensors that can be rapidly and easily introduced into any mouse, which are coupled with intravital microscopy, a powerful tool that can be used to collect cellular and subcellular data from living tissues. Demonstrated application was the observation of in vivo beta-cell-specific ROS dynamics using the Grx1-roGFP2 biosensor and calcium signaling using the GcAMP6s biosensor. Abdominal imaging windows (AIW) can extend in vivo observations beyond single-point terminal measurements to collect longitudinal physiological and biosensor data through repeated imaging of the same mice over time. This platform represents a significant advancement in the ability to study beta-cell structure and signaling in vivo, and its portability for use in virtually any mouse model will enable meaningful studies of beta-cell physiology in the endogenous islet niche.","ResourceType":"DeviceEquipmentTechnology","ParentResourceType":"Technology","DefiningManuscriptId":"PMID31186447","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"raghavendra_mirmira@hirnetwork.org","FirstName":"Raghavendra","LastName":"Mirmira","EmailAddress":"rmirmira@iupui.edu","Organization":"Indiana University","Consortia":["CBDS"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"amelia@hirnetwork.org","FirstName":"Amelia","LastName":"Linnemann","EmailAddress":"aklinnem@iu.edu","Organization":"Indiana University","Consortia":["CBDS","OPP"]}],"Vendor":{"id":null,"Name":null,"Url":null},"VendorResourceName":null,"VendorResourceUrl":null,"VendorCatNumber":null,"VendorLotNumber":null,"DateCreated":"2021-04-30T01:28:30.317164+00:00","DateModified":"2022-07-01T03:35:24.6270324+00:00","VersionId":"1","ModelVersion":"4.0","TechnologyData":{"Dependencies":[],"AssayTechnologyData":{"BioSampleType":[],"CellsTestedWith":[],"AssayTarget":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"AssayCategory":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"TechnologyType":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"TechnologyPlatform":null,"CompatibleTools":[]},"DeviceEquipmentTechnologyData":{"CompatibleTools":[],"CellsTestedWith":[],"Placeholder":"Test"},"ServiceTechnologyData":{"CellsTestedWith":[],"Placeholder":"test"},"CodePipelineTechnologyData":{"Placeholder":"Test"}}}