{"Id":"75aab660-4020-4626-9ade-03b6dba94f4d","CanonicalId":"dx.doi.org/10.17504/protocols.io.buw9nxh6","Url":"https://www.protocols.io/view/lc-ms-ms-quantitation-of-insulin-glucagon-somatost-buw9nxh6","Name":"LC-MS/MS quantitation of insulin, glucagon, somatostatin, and C-peptide ","Description":"An LC-MS method using multiple reaction monitoring for quantitation of insulin, C-peptide, glucagon, and somatostatin secretion from human islet populations is described. A 5 min separation was developed using a 2.1 × 30 mm (i.d. x length) C18 column. The method was used to measure secretions from static incubations of human islets from 2 donors. Insulin and C-peptide matched well with reported values of these hormones.\n","ResourceType":"Protocol","ParentResourceType":null,"DefiningManuscriptId":"","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":null,"Name":"Producer","OntologyId":null},"HirnUserId":"michael_roper@hirnetwork.org","FirstName":"Michael","LastName":"Roper","EmailAddress":"roper@chem.fsu.edu","Organization":"Florida State University","Consortia":["CHIB"]}],"Vendor":{"id":null,"Name":"","Url":null},"VendorResourceName":null,"VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2021-12-06T23:03:07.1675849+00:00","DateModified":"2022-07-01T03:36:55.605273+00:00","VersionId":"1","ModelVersion":"4.0","ProtocolData":{"ProtocolType":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"DocumentId":null,"ProtocolSpecies":[],"DocumentTopic":[]}}