{"Id":"af100b57-1e1d-4cb1-8db4-3299dfe1392d","CanonicalId":"","Url":"https://bioconductor.org/packages/CATALYST","Name":"CATALYST R/Bioconductor package","Description":"The R/Bioconductor package implements several data processing steps for the bead-based compensation method to semi-automate spillover correction in mass cytometry (see details in Assay). First, the FCS file containing data on the bead sample is deconvoluted to identify the individual single-antibody-positive bead populations using a new R implementation of the debarcoding algorithm from Zunder et al. 2015. Then the spillover matrix is calculated based on the spillover observed for single-stained populations. Finally, the calculated compensation matrix is applied to the bead and cell samples to remove interfering signals. There is also a Shiny web application to provide an even friendlier user interface to the data processing pipeline.","ResourceType":"CodePipelineTechnology","ParentResourceType":"Technology","DefiningManuscriptId":"PMID29605184","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"bernd_bodenmiller@hirnetwork.org","FirstName":"Bernd","LastName":"Bodenmiller","EmailAddress":"bernd.bodenmiller@imls.uzh.ch","Organization":"University of Zurich","Consortia":["CBDS"]}],"Vendor":{"id":null,"Name":null,"Url":null},"VendorResourceName":null,"VendorResourceUrl":null,"VendorCatNumber":null,"VendorLotNumber":null,"DateCreated":"2019-04-26T01:22:19.5718812+00:00","DateModified":"2022-07-01T03:35:15.7155536+00:00","VersionId":"1","ModelVersion":"4.0","TechnologyData":{"Dependencies":[],"AssayTechnologyData":{"BioSampleType":[],"CellsTestedWith":[],"AssayTarget":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"AssayCategory":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"TechnologyType":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"TechnologyPlatform":null,"CompatibleTools":[]},"DeviceEquipmentTechnologyData":{"CompatibleTools":[],"CellsTestedWith":[],"Placeholder":"Test"},"ServiceTechnologyData":{"CellsTestedWith":[],"Placeholder":"test"},"CodePipelineTechnologyData":{"Placeholder":"Test"}}}