{"Id":"b3c52e4d-ee70-430d-913d-344514f02e01","CanonicalId":"","Url":"https://pubmed.ncbi.nlm.nih.gov/32429660/","Name":"Assay using extended-lifetime fluorescence dye for profiling glucose-stimulated and M3 receptor-activated insulin secretion dynamics","Description":"A fluorescence anisotropy competitive immunoassay for online insulin detection from single and grouped islets in a microfluidic system, developed using a piezoelectric pressure-driven fluid delivery system and a squaraine rotaxane fluorophore, SeTau-647, as the fluorescent label for insulin. Due to SeTau-647 having a longer lifetime and higher brightness compared to the previously used Cy5 fluorophore, a 45% increase in the anisotropy range was observed with enhanced signal-to-noise ratio (S/N) of the measurements. This new system was tested by measuring glucose-stimulated insulin secretion from single and groups of murine and human islets.","ResourceType":"AssayTechnology","ParentResourceType":"Technology","DefiningManuscriptId":"PMID32429660","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"michael_roper@hirnetwork.org","FirstName":"Michael","LastName":"Roper","EmailAddress":"roper@chem.fsu.edu","Organization":"Florida State University","Consortia":["CHIB"]}],"Vendor":{"id":null,"Name":"","Url":""},"VendorResourceName":"","VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2021-04-30T01:28:29.6140283+00:00","DateModified":"2022-07-01T03:35:02.3713841+00:00","VersionId":"1","ModelVersion":"4.0","TechnologyData":{"Dependencies":[],"AssayTechnologyData":{"BioSampleType":[{"CanonicalId":"http://purl.bioontology.org/ontology/NCBITAXON/9606","Url":"http://purl.bioontology.org/ontology/NCBITAXON/9606","Name":"human","OntologyId":"NCBITAXON"},{"CanonicalId":"http://purl.bioontology.org/ontology/NCBITAXON/10090","Url":"http://purl.bioontology.org/ontology/NCBITAXON/10090","Name":"mouse","OntologyId":"NCBITAXON"}],"CellsTestedWith":[],"AssayTarget":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"AssayCategory":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"TechnologyType":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"TechnologyPlatform":"","CompatibleTools":[]},"DeviceEquipmentTechnologyData":{"CompatibleTools":[],"CellsTestedWith":[],"Placeholder":"Test"},"ServiceTechnologyData":{"CellsTestedWith":[],"Placeholder":"test"},"CodePipelineTechnologyData":{"Placeholder":"Test"}}}