{"Id":"ec27b569-b57b-475a-8c40-a36a25bd64a6","CanonicalId":"US2020/026264","Url":"","Name":"CRISPR-Cas9 conjugation platform for engineering designer beta cells","Description":"This technology relies on newly described chemical modifications that allow site-specific and multiple-site conjugation of a wide assortment of molecules on both the termini and internal sites of Cas9, creating a platform for endowing Cas9 with diverse functions. Using this platform, Cas9 can be modified to more precisely incorporate exogenously supplied single-stranded oligonucleotide donor (ssODN) at the DNA break site. We demonstrate that the multiple-site conjugation of ssODN to Cas9 significantly increases the efficiency of precision genome editing, and such a platform is compatible with ssODNs of diverse lengths.","ResourceType":"DeviceEquipmentTechnology","ParentResourceType":"Technology","DefiningManuscriptId":"PMID32792475","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"bridget_wagner@hirnetwork.org","FirstName":"Bridget","LastName":"Wagner","EmailAddress":"bwagner@broadinstitute.org","Organization":"Broad Institute","Consortia":["CTAR"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"jeff_karp@hirnetwork.org","FirstName":"Jeff","LastName":"Karp","EmailAddress":"jeffkarp@mit.edu","Organization":null,"Consortia":["CHIB"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"amit_choudhary@hirnetwork.org","FirstName":"Amit","LastName":"Choudhary","EmailAddress":"achoud@broadinstitute.org","Organization":"Broad Institute","Consortia":["CTAR"]}],"Vendor":{"id":null,"Name":"","Url":null},"VendorResourceName":null,"VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2021-04-30T01:28:30.3015334+00:00","DateModified":"2022-07-01T03:35:24.6236015+00:00","VersionId":"1","ModelVersion":"4.0","TechnologyData":{"Dependencies":[],"AssayTechnologyData":{"BioSampleType":[],"CellsTestedWith":[],"AssayTarget":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"AssayCategory":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"TechnologyType":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"TechnologyPlatform":null,"CompatibleTools":[]},"DeviceEquipmentTechnologyData":{"CompatibleTools":[],"CellsTestedWith":[],"Placeholder":"Test"},"ServiceTechnologyData":{"CellsTestedWith":[],"Placeholder":"test"},"CodePipelineTechnologyData":{"Placeholder":"Test"}}}