{"Id":"ef5a6afd-9114-4fff-b60e-f9b8e41b5df1","CanonicalId":"","Url":"https://pubmed.ncbi.nlm.nih.gov/30843680/","Name":"Boosting to Amplify Signal with Isobaric Labeling (BASIL) Strategy for comprehensive quantitative phosphoproteomic characterization of small populations of cells","Description":"Phosphoproteomic analysis of small cell populations is a challenge due to insufficient MS signal intensity, and the BASIL innovation of using isobaric labeling to \"\"boost\"\" a sample (e.g., a biological sample mimicking the study samples but available in a much larger quantity) in multiplexed analysis enables sensitive and comprehensive quantitative phosphoproteomic measurements with <100\u202F000 cells. The BASIL strategy increases the overall number of quantifiable phosphorylation sites more than 4-fold. Good reproducibility in quantification was demonstrated with a median CV of 15.3% and Pearson correlation coefficient of 0.95 from biological replicates. A proof-of-concept experiment demonstrated the ability of BASIL to distinguish acute myeloid leukemia cells based on the phosphoproteome data. Moreover, in a pilot application, this strategy enabled quantitative analysis of over 20\u202F000 phosphorylation sites from human pancreatic islets treated with interleukin-1β and interferon-γ. Together, this signal boosting strategy provides an attractive solution for comprehensive and quantitative phosphoproteome profiling of relatively small populations of cells where traditional phosphoproteomic workflows lack sufficient sensitivity.","ResourceType":"AssayTechnology","ParentResourceType":"Technology","DefiningManuscriptId":"PMID30843680","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"jing_chen@hirnetwork.org","FirstName":"Jing","LastName":"Chen","EmailAddress":"jic24@pathology.ufl.edu","Organization":"University of Florida","Consortia":["CMAI"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"tujin_shi@hirnetwork.org","FirstName":"Tujin","LastName":"Shi","EmailAddress":"tujin.shi@pnnl.gov","Organization":"Pacific Northwest National Laboratory","Consortia":["CBDS"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"marina_gritsenko@hirnetwork.org","FirstName":"Marina","LastName":"Gritsenko","EmailAddress":"marina.gritsenko@pnnl.gov","Organization":"Pacific Northwest National Laboratory","Consortia":[]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"mark_atkinson@hirnetwork.org","FirstName":"Mark","LastName":"Atkinson","EmailAddress":"atkinson@pathology.ufl.edu","Organization":"University of Florida","Consortia":["CBDS","HPAC","OPP"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"clayton_mathews@hirnetwork.org","FirstName":"Clayton","LastName":"Mathews","EmailAddress":"cxm@ufl.edu","Organization":"University of Florida","Consortia":["CMAI","CBDS","OPP"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"rohit_kulkarni@hirnetwork.org","FirstName":"Rohit","LastName":"Kulkarni","EmailAddress":"rohit.kulkarni@joslin.harvard.edu","Organization":"Joslin Diabetes Center","Consortia":["CBDS","CTAR"]},{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"wei-jun_qian@hirnetwork.org","FirstName":"Wei-Jun","LastName":"Qian","EmailAddress":"weijun.qian@pnnl.gov","Organization":"Pacific Northwest National Laboratory","Consortia":["CBDS","OPP"]}],"Vendor":{"id":null,"Name":"","Url":""},"VendorResourceName":"","VendorResourceUrl":"","VendorCatNumber":"","VendorLotNumber":"","DateCreated":"2019-04-26T01:22:19.1197314+00:00","DateModified":"2022-07-01T03:35:02.368401+00:00","VersionId":"1","ModelVersion":"4.0","TechnologyData":{"Dependencies":[{"CanonicalId":"http://purl.obolibrary.org/obo/ERO_0002176","Url":"http://purl.obolibrary.org/obo/ERO_0002176","Name":"isobaric labeling","OntologyId":"ERO"}],"AssayTechnologyData":{"BioSampleType":[],"CellsTestedWith":[{"CanonicalId":"http://purl.obolibrary.org/obo/CL_0001064","Url":"http://purl.obolibrary.org/obo/CL_0001064","Name":"malignant cell","OntologyId":"CL"}],"AssayTarget":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"AssayCategory":{"CanonicalId":"http://purl.obolibrary.org/obo/OBI_0000470","Url":"http://purl.obolibrary.org/obo/OBI_0000470","Name":"mass spectrometry assay","OntologyId":"OBI"},"TechnologyType":{"CanonicalId":"","Url":"","Name":"","OntologyId":""},"TechnologyPlatform":"","CompatibleTools":[]},"DeviceEquipmentTechnologyData":{"CompatibleTools":[],"CellsTestedWith":[],"Placeholder":"Test"},"ServiceTechnologyData":{"CellsTestedWith":[],"Placeholder":"test"},"CodePipelineTechnologyData":{"Placeholder":"Test"}}}