{"Id":"f4bf06d6-0f43-416a-9fe6-17f803168943","CanonicalId":"doi:10.17504/protocols.io.bv56n89e","Url":"https://dx.doi.org/10.17504/protocols.io.bv56n89e","Name":"Flow CyTOF Using Single Cells From Human Islets","Description":"Cytometry by time-of-flight (CyTOF) is an application of mass cytometry using antibodies conjugated to rare heavy metal isotopes combined with mass spectrometry. This technique significantly reduces background signals and spectral overlap often seen with traditional fluorescence methods. This protocol describes a technique to analyze multiple cellular features of human islets in parallel using a defined and optimized antibody cocktail.","ResourceType":"Protocol","ParentResourceType":null,"DefiningManuscriptId":"31127054","Publications":[],"Contributors":[{"Role":{"CanonicalId":"hirn-cv-ResourceRole-producer","Url":"resources.hirnetwork.org/api/v1/CV/","Name":"Producer","OntologyId":"HIRN"},"HirnUserId":"klaus_kaestner@hirnetwork.org","FirstName":"Klaus","LastName":"Kaestner","EmailAddress":"kaestner@mail.med.upenn.edu","Organization":"University of Pennsylvania","Consortia":["CTAR","HPAC","OPP","CBDS"]}],"Vendor":{"id":null,"Name":null,"Url":null},"VendorResourceName":null,"VendorResourceUrl":null,"VendorCatNumber":null,"VendorLotNumber":null,"DateCreated":"2021-08-24T16:36:57.8789591+00:00","DateModified":"2022-07-01T03:36:55.5940787+00:00","VersionId":"1","ModelVersion":"4.0","ProtocolData":{"ProtocolType":{"CanonicalId":null,"Url":null,"Name":null,"OntologyId":null},"DocumentId":null,"ProtocolSpecies":[],"DocumentTopic":[]}}