Resource Type
Technology / AssayTechnology
Resource Name

Identification of tissue-specific cell death using methylation patterns of circulating DNA
Canonical Identifier
EP3643795A1
Description
A blood test for detection of cell death in specific tissues based on two principles: (i) dying cells release fragmented DNA to the circulation, and (ii) each cell type has a unique DNA methylation pattern. The authors have identified tissue-specific DNA methylation markers and developed a method for sensitive detection of these markers in plasma or serum. The assay has been used for identification of pancreatic beta-cell death in type 1 diabetes, oligodendrocyte death in relapsing multiple sclerosis, brain cell death in patients after traumatic or ischemic brain damage, and exocrine pancreas cell death in pancreatic cancer or pancreatitis.
Contributors
NameOrganizationConsortiumContact
Clive WasserfallUniversity of Florida Contact
Desmond SchatzUniversity of FloridaCBDS Contact
Carla GreenbaumBenaroya Research InstituteCBDS Contact
Markus GrompeOregon Health and Science UniversityCTAR Contact
Benjamin GlaserHadassah-Hebrew UniversityCTAR Contact
Ruth ShemerHebrew University of JerusalemCBDS Contact
Yuval DorHebrew University of JerusalemCBDS, OPP Contact
Tags or Keywords
Defining Manuscript Identifier
PMID26976580
Related Resources
Canonical IdentifierNameResource Type
Matlab script used for data processing of amplicon bisulfite sequencing reads for selected methylation markers from circulating DNACodePipelineTechnology
Methylome data for human pancreatic alpha cells (two replicates), pancreatic beta cells (three replicates), pancreatic duct cells, and pancreatic acinar cellsDataset


Have you used this resource?


BioSample Type
Cells Tested With
Assay Category



.