Microelectrode arrays (MEAs) can be used to better understand the electrophysiology of dissociated islet cells in response to glucose in a real-time, non-invasive method over prolonged culture periods. Human islets dissociated into singular cells can be seeded onto MEA and cultured for up to 7 days. Immunofluorescent imaging revealed that several cellular subtypes of islets remained present after dissociation. At days 1, 3, 5, and 7 of culture, MEA recordings can capture higher electrical activities of islet cells under different glucose concentration conditions, e.g. high glucose vs. low glucose. MEA spiking profiles were similar to the time course of insulin response when glucose concentration is switched from low to high. Extracellular recordings of dissociated islet cells using MEA is an effective approach to rapidly assess islet functionality, and could supplement standard assays such as glucose stimulate insulin response.