Resource Type
Technology / DeviceEquipmentTechnology
Resource Name

Microfluidic human islet on a chip designed for scalable manufacturing with synchronized stimulation and continuous insulin sensing

Canonical Identifier
Description
A thermoplastic, microfluidic-based islet-on-a-chip compatible with commercial fabrication methods, that automates islet loading, stimulation, and insulin sensing. Inspired by the perfusion of native islets by designated arterioles and capillaries, the chip delivers synchronized glucose pulses to islets positioned in parallel channels. By flowing suspensions of human cadaveric islets onto the chip, we confirmed automatic capture of islets. Fluorescent glucose tracking demonstrated that stimulus delivery was synchronized within a two-minute window independent of the presence or size of captured islets. Insulin secretion was continuously sensed by an automated, on-chip immunoassay and quantified by fluorescence anisotropy.
Contributors
NameOrganizationConsortiumContact
Ben PopeHarvard University Contact
Douglas MeltonHarvard UniversityCHIB, CBDS, CMAI Contact
Michael RoperFlorida State UniversityCHIB Contact
Defining Manuscript Identifier


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