Interrogating cell-type specific chromatin accessibility can reveal cis-regulatory elements linked to downstream gene expression patterns. Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-Seq) is a technique that assays genome-wide chromatin accessibility using a tagmentation protocol that inserts sequencing adapters into open genomic regions. This technique can reveal nucleosome positioning patterns, map enhancer and promoter regions, or reveal transcription factor binding sites. When applied to studies of diabetic patients, this technique can reveal potential differences in genomic accessibility patterns between non-diabetic pancreata compared to diseased organs.