Imaging mass cytometry (IMC) is a multiplexed imaging method that allows samples to be probed with up to forty antibodies at one time. This enables visualization of multiple cell types simultaneously in the native tissue microenvironment. For example, IMC can be applied to study the interactions between endocrine cells and the immune system in pancreata from donors diagnosed with type 1 diabetes. This protocol describes a technique for IMC staining of paraffin sections with a defined antibody panel to identify immune, islet, endocrine, and stromal cell types of the pancreas.