Resource Type
Protocol
Resource Name

Functional Characterization of the Human Islet Microvasculature Using Living Pancreas Slices

Canonical Identifier
Description
Protocol for characterization of living pancreas slices from non-diabetic human donors to examine the function of human islet blood vessels. Living human pancreas slices were incubated with a membrane permeant calcium indicator and pericytes/smooth muscle cells were visualized with a fluorescent antibody against the mural cell marker NG2 proteoglycan. By confocal microscopy, we simultaneously recorded changes in the diameter of lectin-labeled blood vessels and cytosolic calcium levels in mural cells in islets. We tested several stimuli with vasoactive properties, such as norepinephrine, endothelin-1 and adenosine and compared human vascular responses with those previously published for mouse islet blood vessels.
Contributors
NameOrganizationConsortiumContact
Joana AlmacaUniversity of MiamiHPAC, OPP Contact
Defining Manuscript Identifier


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