There has been an increasing interest in the sources of hematopoietic and mesenchymal stromal (stem) cells (MSCs) and their respective subpopulation from the human bone marrow. Vertebral bodies (VBs) from deceased donors represent a useful source for large scale production of the above mentioned cell products for clinical and research applications. The Standard Operating Procedure (SOP) utilized at the cGMP Human Cell Processing Facility of the Cell Transplant Center and Diabetes Research Institute at the University of Miami is presented below. This SOP has been used to manufacture cellular products for clinical and research applications at the University of Miami and at remote sites as part of cellular product distribution programs, including NIH clinical trials for tolerance induction in organ transplantation.