Resource Type
Protocol
Resource Name

Human Islet Sorting for Alpha, Beta, and Acinar Cells
Canonical Identifier
doi:10.17504/protocols.io.bv9xn97n
Description
Fluorescence-activated cell sorting (FACS) relies on cell-type specific properties to isolate individual populations from a heterogenous sample. This protocol for human islet sorting allows for separation of α-cells, β-cells, and acinar cells from donor pancreata for downstream analyses, such as genomic assays, electrophysiology, and single-cell calcium imaging.
Contributors
NameOrganizationConsortiumContact
Klaus KaestnerUniversity of PennsylvaniaCTAR, HPAC, OPP, CBDS Contact
Defining Manuscript Identifier
31127054


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