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Search Results for: Chunhua Dai returned 92 results
Gcgr-/- MGI:3505881 Mice lacking glucagon receptor have been characterized with mild hypoglycemia, alpha-cell hyperplasia and hypertrophy, and altered responses to intraperitoneal glucose and insulin tolerance tests. Pups born from Gcgr-/- mothers die perinatally. |
ST5 antibody RRID:AB_10861960 DISCONTINUED. |
G6pc2-KO (C57BL/6J background) G6pc2 KO mice on a mixed 129SvEvBRD x C57BL/6J and then backcrossed onto pure C57BL/6J. (Acute treatment with dexamethasone selectively induces endogenous G6pc2 expression in 129SvEv but not C57BL/6J mouse pancreas and isolated islets.) |
B6 ob RRID:IMSR_JAX:000632 From Jackson Labs: Mice homozygous for the obese spontaneous mutation, Lepob (commonly referred to as ob or ob/ob), exhibit obesity, hyperphagia, transient hyperglycemia, glucose intolerance, and elevated plasma insulin. They are also hypometabolic, hypothermic, and subfertile. Wound healing is impaired and hormone production from both pituitary and adrenal glands is increased. This strain is used to model phases I and II of diabetes type II and obesity. Obesity is characterized by an increase in the number and size of adipocytes. Although hyperphagia contributes to the obesity, homozygotes gain excess weight and deposit excess fat even when restricted to a diet sufficient for normal weight maintenance in lean mice. |
G6pc2-KO (129SvEvBRD background) G6pc2 KO mice on a mixed 129SvEvBRD x C57BL/6J and then backcrossed onto pure 129SvEvBRD. (Acute treatment with dexamethasone selectively induces endogenous G6pc2 expression in 129SvEv but not C57BL/6J mouse pancreas and isolated islets.) In 6-hour fasted, nonstressed 129SvEv mice, deletion of G6pc2 lowers fasting blood glucose (FBG). In response to the stress of repeated physical restraint, which is associated with elevated plasma glucocorticoid levels, G6pc2 gene expression is induced and the difference in FBG between wild-type and knockout mice is enhanced. |
Whole Cell Patch Clamp of Dispersed Human Islet Cells dx.doi.org/10.17504/protocols.io.bv3un8nw Cells use exocytosis to secrete a wide variety of molecules, including proteins, hormones, and neurotransmitters. Exocytosis can be monitored at the single-cell level by using patch-clamp electrophysiology to measure changes in membrane capacitance as vesicles fuse with the cell membrane and release their content. Dispersion of pancreatic islets into single cells allows for individual characterization of electrophysiological characteristics and allows for collection of cellular content for recovery of full-length transcriptomes by use of Smart-seq2. Described in this protocol is the dispersion of pancreatic islets into single cells followed by whole-cell patch clamp electrophysiology which includes parameters representing cell size, exocytosis, sodium channel currents, and calcium channel currents. Cells are then collected individually after recording to be processed for single-cell RNA sequencing. |
INS-1 832/13 RRID:CVCL_7226 INS-1-derived cell line with robust ATP-sensitive K+ channel-dependent and -independent glucose-stimulated insulin secretion (Hohmeier et al., 2000) |
Human/Mouse/Bovine Insulin APC-conjugated Antibody RRID:AB_2126535 |
Beta-TC-3 RRID:CVCL_0172 Islet-derived immortalized cell line. |
Anti-Glucagon Antibody RRID:AB_433707 |
Cy™5 AffiniPure Donkey Anti-Sheep Antibody RRID:AB_2340730 |
Ins2-rtTA Strain allows for transgene induction of p16 using the Ins2-rtTA driver (tet on). Mice shows decreased overall beta cell mass. |
Phospho-S6 Ribosomal Protein RRID:AB_10694233 |
Cy™3 AffiniPure Goat Anti-Mouse Antibody RRID:AB_2338680 |
Transcript data of proliferating ?-cell after GCGR mAb treatment; from Gcgr+/+ and Gcgr-/- mice GSE89035 |
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