Search Results for: Desmond Schatz returned 15 results

ImmPRESS Universal Antibody Kit
RRID:AB_2336534

Matlab script used for data processing of amplicon bisulfite sequencing reads for selected methylation markers from circulating DNA

A script for filtering and identifying reads (from Illumina) for specific methylation markers.

NGS targeted sequencing of 140 diabetes/diabetes-related genes from 38 donor DNA samples that met clinical inclusion for possible monogenic diabetes
Request
(Data not deposited/available upon request.)

Identification of tissue-specific cell death using methylation patterns of circulating DNA
EP3643795A1
A blood test for detection of cell death in specific tissues based on two principles: (i) dying cells release fragmented DNA to the circulation, and (ii) each cell type has a unique DNA methylation pattern. The authors have identified tissue-specific DNA methylation markers and developed a method for sensitive detection of these markers in plasma or serum. The assay has been used for identification of pancreatic beta-cell death in type 1 diabetes, oligodendrocyte death in relapsing multiple sclerosis, brain cell death in patients after traumatic or ischemic brain damage, and exocrine pancreas cell death in pancreatic cancer or pancreatitis.

Methylome data for human pancreatic alpha cells (two replicates), pancreatic beta cells (three replicates), pancreatic duct cells, and pancreatic acinar cells

IHC‑plus™ Polyclonal Rabbit anti‑Human WFS1 Antibody
RRID:AB_2938618

Multiplex DNA methylation marker assay to detect circulating cell-free DNA derived from human pancreatic beta-cells

This ultrasensitive assay allows for detection of a beta cell-specific DNA methylation signature, by simultaneous assessment of 6 DNA methylation markers, that identifies beta cell DNA in mixtures containing as little as 0.03% beta cell DNA (less than 1 beta cell genome equivalent). Based on this assay, plasma from nondiabetic individuals (N = 218, aged 4-78 years) contained on average only 1 beta cell genome equivalent/mL. As expected, cell-free DNA (cfDNA) from beta cells was significantly elevated in islet transplant recipients shortly after transplantation. The assay also detected beta cell cfDNA in a patient with KATP congenital hyperinsulinism, in which substantial beta cell turnover is thought to occur.

PERK/EIF2AK3 Polyclonal Antibody
RRID:AB_2879521

RNA-seq from laser microdissected islets in 8 non-diabetic, 3 autoantibody positive, and 7 T1D donors
GSE181674
Fresh frozen pancreas sections were obtained from non-diabetic autoantibody-negative controls (N=8) and autoantibody-positive (N=3) donors and patients with T1D (N=7). Islet laser microdissection was performed followed by RNA isolation and RNA-sequencing or Nanostring nCounter analyses.

Anti-GATA-4 Antibody
RRID:AB_627667

Genotyping data of diabetes-risk variants for 1,946 research participants from the University of Florida Diabetes Institute (UFDI) cohort
Request
(Data not deposited/available upon request.) Includes control subjects [type 1 diabetes-unaffected and non-first- or -second-degree relatives of type 1 diabetes patients] (n = 405), first-degree relatives with ≤1 type 1 diabetes-relevant autoantibody (AAb) (n = 790), second-degree relatives with ≤1 AAb (n = 68), multiple AAb positive at-risk relatives (≥2AAb + , n = 46), and type 1 diabetes patients (n = 637). The typed loci are those curated on immunobase.org as of October 2017.

Anti-GLIS3 Antibody
RRID:AB_2683128

IHC‑plus™ Monoclonal Mouse anti‑Human STAT5B Antibody
RRID:AB_10915294

Polyclonal Rabbit Anti-Human Somatostatin antibody
RRID:AB_2688022

Mouse Anti-Glucagon Monoclonal Antibody, Unconjugated, Clone K79bB10
RRID:AB_297642

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