Clear Filters
Resource Type ▲
- Antibody (24)
- ModelOrganism (8)
- AssayTechnology (1)
- Dataset (1)
- Protocol (1)
- SoftwareDatabaseTechnology (1)
Usage / Applications ▼
- immunohistochemistry (13)
- immunofluorescence microscopy assay (6)
- immuno staining assay (4)
- western blot assay (4)
- humanized mouse (3)
- immunocytochemistry (3)
- transgenic organism (3)
- confocal fluorescence microscopy (2)
- flow cytometry assay (2)
- phenotype (2)
- TCR:BCR Tool (1)
- cryosectioning (1)
- genotyping (1)
- immunofluroscence micorscopy assay (1)
- immunoh (1)
- western blot assay (1)
Contributor Organization ▼
- University of Miami (20)
- University of Florida (3)
- Vanderbilt University (3)
- Harvard University (2)
- Oregon Health and Science University (2)
- University of Geneva (2)
- University of Pennsylvania (2)
- City of Hope (1)
- Eastern Virginia Medical School (1)
- Indiana University School of Medicine (1)
- University of Tennessee (1)
Search Results for: Joana Almaca returned 36 results
Functional Characterization of the Human Islet Microvasculature Using Living Pancreas Slices doi:10.17504/protocols.io.bwgupbww Protocol for characterization of living pancreas slices from non-diabetic human donors to examine the function of human islet blood vessels. Living human pancreas slices were incubated with a membrane permeant calcium indicator and pericytes/smooth muscle cells were visualized with a fluorescent antibody against the mural cell marker NG2 proteoglycan. By confocal microscopy, we simultaneously recorded changes in the diameter of lectin-labeled blood vessels and cytosolic calcium levels in mural cells in islets. We tested several stimuli with vasoactive properties, such as norepinephrine, endothelin-1 and adenosine and compared human vascular responses with those previously published for mouse islet blood vessels. |
Expansion of Islet-Resident Macrophages Leads to Inflammation Affecting Beta Cell Proliferation and Function in Obesity GSE112002 |
Brilliant Violet 605™ anti-mouse CD206 (MMR) Antibody RRID:AB_2562340 |
ACE Polyclonal Antibody RRID:AB_2745827 |
APC anti-human CD45 Antibody RRID:AB_314399 |
Anti-Serotonin Antibody RRID:AB_477522 |
Functional characterization of the human islet microvasculature using living pancreas slices This describes an assay for characterization of living pancreas slices, where slices were incubated with a membrane permeant calcium indicator and pericytes/smooth muscle cells were visualized with a fluorescent antibody against the mural cell marker NG2 proteoglycan. Confocal microscopy can be used to simultaneously record changes in the diameter of lectin-labeled blood vessels and cytosolic calcium levels in mural cells in islets. Several stimuli with vasoactive properties can be tested, such as norepinephrine, endothelin-1 and adenosine, to compare human vascular responses with those previously published for mouse islet blood vessels. Data from the assay are published in the indicated defining manuscript. |
Human NG2/MCSP Alexa Fluor® 647-conjugated Antibody RRID:AB_3648561 |
PE anti-human CD14 Antibody RRID:AB_314187 |
B6;129S6-Gt(ROSA)26Sortm96(CAG-GCaMP6s)Hze/J RRID:IMSR_JAX:024106 |
B6(Cg)-Tg(Phox2b-cre)3Jke/J RRID:IMSR_JAX:016223 |
Anti-Substance P Antibody RRID:AB_94639 |
Calcitonin Gene Related Peptide Polyclonal Antibody RRID:AB_2068527 |
Anti-NeuN Antibody RRID:AB_10918200 |
B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J RRID:IMSR_JAX:007909 |
.