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Search Results for: Martha Campbell Thompson returned 83 results
ImmPRESS Universal Antibody Kit RRID:AB_2336534 |
Mouse Anti-Actin, alpha-Smooth Muscle Monoclonal Antibody, Cy3 Conjugated, Clone 1A4 RRID:AB_476856 |
Human Pancreas Histopathology Assessment https://dx.doi.org/10.17504/protocols.io.br7tm9nn The purpose of this protocol is to outline proper procedures for fixed specimen processing using an automatic paraffin processor and sectioning and staining for histopathological review of normalcy. |
Human Pancreas Optical Clearing by iDISCO and VISIKOL dx.doi.org/10.17504/protocols.io.ba4qigvw Optical clearing of tissues to improve deep tissue microscopy was described over a century ago and methods have improved considerably following publication of the CLARITY method from Karl Deisseroth in 2013. Improvements in organic and inorganic methods are constantly reported for multiple species and organs. Large volume imaging was needed for human pancreas studies due to known heterogeneity in normal islet sizes and proportions of endocrine cells. Lobularity in islet beta-cell loss and islet inflammation is also seen in patients with type 1 diabetes. The iDISCO protocol allows for rapid clearing, easy staining, and high resolution confocal imaging. Human pancreas samples were cleared using iDISCO as originally published. This protocol describes steps to clear 4mm x 6mm x 400um deep sections of 4% paraformaldehyde fixed human pancreas using iDISCO-like reagents purchased from Visikol. |
R-Phycoerythrin AffiniPure F(ab')₂ Fragment Goat Anti-Mouse IgM, µ chain specific Antibody RRID:AB_2338628 |
CD45 Monoclonal Antibody RRID:AB_1603240 |
CD 3 (Pan-T) antibody RRID:AB_2335677 |
CD105 Antibody RRID:AB_10827601 |
HPa3 / HIC3-2D12 antibody RRID:AB_2631139 |
CD34 Monoclonal Antibody (QBEND/10) RRID:AB_11156010 |
Fluorescein (FITC) AffiniPure™ Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ Fragment Specific Antibody RRID:AB_2338600 |
Proteomics of laser dissected islets from T1D and control donors from the Network for Pancreatic Organ Donors with Diabetes (nPOD) and 10-100 cultured HeLa cells PXD006847 |
Anti-SCG3 antibody produced in rabbit RRID:AB_1079898 |
Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody RRID:AB_2535739 |
Allophycocyanin-AffiniPure F(ab')2 Fragment Donkey Anti-Chicken IgY (IgG) (H+L) (min X Bov,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot) antibody RRID:AB_2340360 |
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