Search Results for: Peter Buchwald returned 19 results

Local concentration-based finite element method (FEM) computational model

This FEM multiphysics model can be used for the quantitative characterization of the insulin response of cultured, perifused, transplanted, or encapsulated islets. It has been experimentally validated with free and alginate microencapsulated islet in high-resolution GSIR perifusion experiments. The model is implemented in COMSOL Multiphysics 4.4.

C57BL/6J
RRID:IMSR_JAX:000664
C57BL/6J is the most widely used inbred strain and the first to have its genome sequenced. Although this strain is refractory to many tumors, it is a permissive background for maximal expression of most mutations. C57BL/6J mice are resistant to audiogenic seizures, have a relatively low bone density, and develop age related hearing loss. They are also susceptible to diet-induced obesity, type 2 diabetes, and atherosclerosis. Macrophages from this strain are resistant to the effects of anthrax lethal toxin.

FP-3W (3-well fluidific platform)
RRID:SCR_015808
The FP-3W (3-well fluidific platform) is a re-sealable, customizable platform that was optimized for perfusion assays of rodent and human pancreatic islets; it has demonstrated use for GSIS with concomitant live-cell imaging, and optogenetic experiments. Notably, it can be disassembled to retrieve islets for post-processing, and may be suitable as well for long-term culture of islets. Compared with other commercial platforms, the FP-3W has reduced response delay, is more user friendly (simpler), and can be suggested for drug testing because it is PDMS-free. The FP-3W can be integrated with existing instruments such as the PERI4-02 perfusion station and an inverted microscope for imaging.

NOD.129S7(B6)-Rag1tm1Mom/J
RRID:IMSR_JAX:003729

DBA/2J
RRID:IMSR_JAX:000671

BALB/cJ
RRID:IMSR_JAX:000651
BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO).

Rag1 KO
RRID:IMSR_JAX:002216
From Jackson Labs: Mice homozygous for the Rag1tm1Mom mutation produce no mature T cells or B cells. Their phenotype can be described as a \non-leaky\\\\"\\\" immune deficiency.\\\"\\\"

Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody
RRID:AB_141882

NOD-scid
RRID:IMSR_JAX:001303
From Jackson Labs: Mice homozygous for the severe combined immune deficiency spontaneous mutation Prkdcscid, commonly referred to as scid, are characterized by an absence of functional T cells and B cells, lymphopenia, hypogammaglobulinemia, and a normal hematopoietic microenvironment. Prkdcscid mice accept allogeneic and xenogeneic grafts making them an ideal model for cell transfer experiments. In general, Prkdcscid leakiness is low on the NOD/ShiLtSz genetic background.

Human/Mouse/Bovine Insulin APC-conjugated Antibody
RRID:AB_2126535

Ingenuity Pathway Analysis (IPA) software package
RRID:SCR_008653

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody
RRID:AB_2576217

Lipidomics in high-risk subjects for the identification of integrated biomarker signatures of type 1 diabetes
doi:10.21228/M8ZX18
Lipidomics in high-risk subjects for the identification of integrated biomarker signatures of type 1 diabetes

Metabolic profiling of isolated human islets with inflammation and hypoxia under basal and hyperglycemic conditions

Metabolomic data of human blood samples for T1D biomarker identification
doi:10.21228/M8B123
Untargeted metabolomic analysis of human blood samples via qualitative GC-MS for T1D biomarker identification

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