Resource Type
Technology / AssayTechnology
Resource Name

CellCycleTRACER (mass cytometry assay method for deconfounding cell-cycle and cell-volume effects)

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Description
CellCycleTRACER is a combined experimental and computational method to account for the often confounded factors cell cycle and cell volume in mass cytometry data. Resolving cell heterogeneity caused by either cell-cycle state or cell volume in single-cell transcriptomic data has relied on statistical analysis using known cell-cycle genes. For mass cytometry, there is so far a more limited selection of available informative markers related to cell cycle and volume. As an assay method, CellCycleTRACER exploits four cell-cycle markers recently identified by Behbehani et al. 2012 (phosphorylated histone H3, phosphorylated retinoblastoma, cyclin B1, and 5-Iodo-2′-deoxyuridine) to distinguish cell populations in different cell-cycle states. For measuring cell volume, CellCycleTRACER uses the ruthenium complex ASCQ_Ru. CellCycleTRACER may be particularly important when heterogeneous cell populations with deregulated cellular processes, as typically found in tumors, are analyzed.
Contributors
NameOrganizationConsortiumContact
Bernd BodenmillerUniversity of ZurichCBDS Contact
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