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Technology / AssayTechnology
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Assay using extended-lifetime fluorescence dye for profiling glucose-stimulated and M3 receptor-activated insulin secretion dynamics

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Description
A fluorescence anisotropy competitive immunoassay for online insulin detection from single and grouped islets in a microfluidic system, developed using a piezoelectric pressure-driven fluid delivery system and a squaraine rotaxane fluorophore, SeTau-647, as the fluorescent label for insulin. Due to SeTau-647 having a longer lifetime and higher brightness compared to the previously used Cy5 fluorophore, a 45% increase in the anisotropy range was observed with enhanced signal-to-noise ratio (S/N) of the measurements. This new system was tested by measuring glucose-stimulated insulin secretion from single and groups of murine and human islets.
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NameOrganizationConsortiumContact
Michael RoperFlorida State UniversityCHIB Contact
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