Resource Type
Technology / DeviceEquipmentTechnology
Resource Name

A microfluidic platform integrating pressure-driven and electroosmotic-driven flow with inline filters for affinity separations

Canonical Identifier
Description
A microfluidic system that incorporates an online non-competitive assay for glucagon with a 6 pM limit of detection, low enough to measure from a single islet. It was developed to maintain appropriate flow rates in the presence of the high concentration of salt required for the assay through the use of in-line flow sensors. Incorporation of in-line filters allow operation the device for more than 4 hours without clogs. Use of this system enabled highly reproducible injections. Other engineered features improve the utility of device for long-term monitoring of the glucagon release from islets, but has the potential to be applied to a number of other microfluidic separation-based assays as well.
Contributors
NameOrganizationConsortiumContact
Michael RoperFlorida State UniversityCHIB Contact
Defining Manuscript Identifier


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