Resource Type
Protocol
Resource Name

Generation of Immunodeficient Mice Bearing Human Immune Systems by the Engraftment of Hematopoietic Stem Cells
Canonical Identifier
doi:10.17504/protocols.io.bu9nnz5e
Description
This is a standard protocol for the engraftment of human HSC into 21-day-old NOD-scid IL2rγ (NSG) mice using an intravenous injection approach. Also described is the multiparameter flow cytometry used to monitor human immune system development and the kinetics of development.
Contributors
NameOrganizationConsortiumContact
Michael BrehmUniversity of Massachusetts Medical SchoolCMAI Contact
Dale GreinerUniversity of Massachusetts Medical SchoolCMAI Contact
Leonard ShultzThe Jackson LaboratoryCMAI Contact
Tags or Keywords
Defining Manuscript Identifier
PMID27150084


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