Interrogating cell-type specific chromatin accessibility can reveal cis-regulatory elements linked to downstream gene expression patterns. Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-Seq) is a technique that assays genome-wide chromatin accessibility and can reveal nucleosome positioning patterns, map enhancer and promoter regions, or reveal transcription factor binding sites. Single-cell Assay for Transposase-Accessible Chromatin using Sequencing (scATAC-seq) adapts the bulk ATAC-sequencing protocol to separate individual nuclei and assay chromatin state with single-cell resolution. This technique allows for resolution of gene accessibility patterns within specific pancreatic cell types such as α-cells, β-cells, and acinar cells.