Resource Type
Protocol
Resource Name

Flow CyTOF Using Single Cells From Human Islets

Canonical Identifier
Description
Cytometry by time-of-flight (CyTOF) is an application of mass cytometry using antibodies conjugated to rare heavy metal isotopes combined with mass spectrometry. This technique significantly reduces background signals and spectral overlap often seen with traditional fluorescence methods. This protocol describes a technique to analyze multiple cellular features of human islets in parallel using a defined and optimized antibody cocktail.
Contributors
NameOrganizationConsortiumContact
Klaus KaestnerUniversity of PennsylvaniaCTAR, HPAC, OPP, CBDS Contact
Defining Manuscript Identifier


Have you used this resource?




.